PDet-1 (Peptergent-1) is a detergent-free solubilization system derived from Peptidisc-based membrane mimetic technology, engineered to enable selective membrane protein extraction while preserving native-like stability and complex architecture — without the use of conventional detergents!

• For isolated membrane preparations, PDet-1 is recommended at a 1:1 (w/w) ratio to crude membranes, delivering controlled extraction with typical yields of ~35% relative to the 1% DDM benchmark. These conditions support workflows that prioritize functional integrity, complex preservation, and downstream performance over maximal bulk recovery, while enabling practical, single-digit dollar per-experiment costs through reduced loading.

• For direct whole-cell solubilization, PDet-1 has been validated at working concentrations of approximately 2 mg/mL, reflecting the higher complexity of intact cells and supporting robust extraction under mild, detergent-free conditions.